
Agarose gel electrophoresis is a method to separate DNA or RNA molecules by size.
Biotechnology is the science of making use of living organisms and their constituents to produce services and goods. Biotechnological products are produced by manipulating and modifying organisms, usually at molecular level. It is a vast discipline and has numerous practical applications in agriculture, medicine and industry.
Biotechnology brings together the knowledge, procedures and practices a large array of disciplines including genetics, molecular biology, biochemistry, embryology, cellular biology, biochemistry, immunology, cell biology and more. It also spans across other fields such as chemical engineering, information technology, and robotics.
Just in the territory of agriculture, applications of the field can be witnessed in the form of modified agricultural produce including improve yield from crops, better immunity of crops to environmental stresses, enhanced nutritional qualities of food crops, qualitative improvements in texture, taste or appearance of food items, decreased dependence on pesticides, fertilizers and other agrochemicals, production of new substances in crops, etc.
Perhaps the most fascinating developments of biotechnology have appeared in medicine where it finds applications in pharmacogenomics, genetic testing, pharmaceutical drug production and gene therapy.
As the discipline has evovled different classifications of it based on applications have also surfaced, some of them are:
Green biotechnology: This term refers to the discipline's applications in agricultural processes. An example is growing of food crops with enhanced nutritional content.
Bioinformatics: Bioinformatics is yet another interdisciplinary field that entails solving biological problems using computer applications, and is used to quickly organize and analyze biological data.
Blue biotechnology: This term refers to biotechnology's marine and aquatic applications applications.
Red biotechnology: This one refers to its applications in medical processes. For instance, production of organisms to produce antibiotics, or providing genetic cures by means of genomic manipulation.
White biotechnology: It refers to biotechnology used in industrial processes. For instance designing an organism to produce a needed chemical compound.
All final products derived from biotechnological processes and any intermediate ingredients used to make the final products are known as biotechnological products.
Types of Biological Products
Antibodies
Also known as immunoglobulins, antibodies are gamma globulin proteins found in blood and several other bodily fluids of vertebrates (including humans). The function of these proteins is to act as a constituent of the immune system by identifying and neutralizing foreign objects, such as bacteria and viruses, that invade our bodies. Antibodies are produced by a type of white blood cell known as a "B cell." Structuraly, comprising heavy chains, antibodies are of many types and are grouped into different isotypes depending upon which heavy chain they comprise.
Assay Systems
The term assay refers to a procedure by which a property or concentration of substance used in a laboratory is measured. A large number for biological and medical purposes exist e.g. competitive protein binding assay, bioassay, antigen capture assay, immunoassay, stem cell assay, microbiological assay, and many more types. For instance typical assays for researching proteins' interactions with DNA include, DNase footprinting assay, filter binding assay, gel shift assay. An example of Assays meant to study how toxic a compound is to cells typically entails MTT assay, SRB (Sulforhodamine B) assay. Assay systems on the other hand refers to tools and kits meant to conduct such an analysis.
Proteins
Proteins are large molecules that assist living organisms in their structure, function, and regulation of the body's organs, tissues and cells. There are different types of proteins and each of which has a specialized function. They are also the main components of bones, muscles, skin, and other parts of the body. Additionally, they also serves as a sources of energy for the body. In case of cells, proteins are the chief actors that govern the myriad cellular acivities specified by the information contained in the genes. Enzymes too are a type of proteins that aid in accomplishing various functions such as metabolism, catabolism, DNA repair, DNA replication, and RNA synthesis. Antibodies are themselves proteins with the funtion of protecting the body by binding them around antigens, or any foreign substances inside the body, and destroy them.